Proteomics researchers are realizing the promise of Data-Independent Acquisition (DIA), but there is a need to lower the barriers to adoption by developing best practices using a common sample. Due to its multitude of options, DIA methods vary from lab to lab. In its 2017/2018 study, the ABRF Proteomics Research Group (PRG) identified the need for a go-to resource that provides information about sample processing, data acquisition and data analysis for new users interested in DIA studies. This need motivated the PRG to undertake a further study along these lines in 2018/2019. This workshop will facilitate in-depth discussion of the PRG study results, and provide practical demonstrations utilizing diverse DIA data sets as well as those created during the PRG study.