(SW9) Hands-on Light Sheet Microscopy

Workshop Sponsors:


Sunday, April 22

8:00 am – 4:00 pm

Please be advised this session will be capped at 20 participants. Don't delay - register today!

Light-sheet fluorescence microscopy (LSFM) is a burgeoning meso to nanoscale advancement in imaging that has revolutionized many imaging applications, especially those requiring 3D datasets in a timely manner.

There is a long history of this powerful imaging modality and a growing number of implementations that all have benefits in certain applications, especially in such fields as Developmental Biology, Cell Biology, Systems Biology, neuroscience, organoid function and Oncology.  Like many advanced imaging technologies, the sophistication, expenses, sample and image processing pipelines are best suited for housing a system (or systems) within a shared core facility environment.

Workshop Goals

This workshop is intended to help introduce participants to the various LSFM embodiments and to help identify the requirements in sample preparation, imaging and cyberinfrastructure to successfully support and grow this powerful research tool in multi-user environments. Participants can expect a broad scientific program covering topics such as:

  • Light-sheet/3D live imaging & fixed cleared applications (variants include Single Plane Illumination Microscopy (SPIM), Digitally Scanned Light-sheet Microscopy (DSLM), Ultramicroscopy, Objective-Coupled Planar Microscopy (OCPI), Oblique Plane Microscopy (OPM), Multidirectional SPIM (mSPIM), Bessel-Beam-based Light-sheet Microscopy, and Individual Molecule Localization SPIM (IML-SPIM).
  • Optical clearing for fixed samples (commercial and home-brewed)
  • Non-light-sheet high-speed, high-resolution techniques
  • Image analysis & big data challenges


Mark A. Sanders, University of Minnesota


Mark A. Sanders, University of Minnesota
Joshua Z. Rappoport, Northwestern University Feinberg School of Medicine
Thomas Pengo, University of Minnesota


Platinum Sponsors of the ABRF